|
|
Methodology for assessing the effect of sublethal doses of pesticides on solitary bees employing the OMICs approach
Erban, Tomáš ; Shcherbachenko, Elena ; Šlachta, Martin ; Cudlín, Pavel ; Chalupníková, Julie ; Halešová, Taťána ; Tomešová, Daniela ; Václavíková, Marta ; Votavová, Alena
Pollinators are potentially exposed to a number of pesticides in the environment. The most important group of hazardous substances comes from the category of plant protection products (PPPs), which are applied not only by farmers but also by small gardeners. Each PPP including the active substance and formulation undergoes a very detailed and thorough evaluation, which is in the European Union (EU) based on Regulation (EC) No 1107/2009 of the European Parliament and of the Council. Although the negative effects of PPPs on the environment and non-target organisms are minimized with proper use, we cannot rule out possible hidden effects, especially those of a sublethal nature. Modern analytical approaches, such as the application of high-throughput methods, help to identify hidden side effects. This methodology is focused on the evaluation of the effect of sublethal doses of pesticides on solitary bees employing the OMICs approach. In the risk assessment of pesticides, the honey bee has long been preferred as a model species of pollinator, while solitary bees stay in the background. The methodology contains innovative aspects for experiments, such as a modified isolator, preparation of plants for exposure, the timing of the experiment, monitoring of active substance distribution or proteomic analysis of solitary bees to identify hidden effects of the test substance or its formulation. In this methodology, the model of the solitary bee, red mason bee (Osmia bicornis), is considered. However, the methodology is also applicable to other, especially related species of solitary bees. The methodology can be used in areas of state administration, private laboratories and research activities in the assessment of environmental risks of pesticides on solitary bees. The methodological procedure can confirm or even eliminate environmental risks when registering new products or reevaluating existing products. This methodology therefore has the potential for use in testing new substances intended for plant protection before their registration. In an exemplary embodiment, the effect of acetamiprid in a Careo sticks formulation was tested. The distribution of acetamiprid in the soil substrate and the plant was evaluated. The hazardous metabolite acetamiprid IM-2-1 was identified in plant tissues. However, proteomic analysis indicated a very low risk of acetamiprid for O. bicornis. This result is in agreement with the fact that acetamiprid has been assessed by EFSA to be a low risk for bees and is registered in the EU until 28 February 2033.
Fulltext:  PDF
|
|
|
Methodology for assessing the effect of sublethal doses of pesticides on solitary bees\nemploying the OMICs approach
Erban, T. ; Shcherbachenko, E. ; Šlachta, Martin ; Cudlín, Pavel ; Chalupníková, J. ; Halešová, T. ; Tomešová, D. ; Václavíková, M. ; Votavová, A.
Pollinators are potentially exposed to a number of pesticides in the environment. The\nmost important group of hazardous substances comes from the category of plant protection\nproducts (PPPs), which are applied not only by farmers but also by small gardeners. Each PPP\nincluding the active substance and formulation undergoes a very detailed and thorough evaluation,\nwhich is in the European Union (EU) based on Regulation (EC) No 1107/2009 of the European\nParliament and of the Council. Although the negative effects of PPPs on the environment and nontarget organisms are minimized with proper use, we cannot rule out possible hidden effects,\nespecially those of a sublethal nature. Modern analytical approaches, such as the application of\nhigh-throughput methods, help to identify hidden side effects. This methodology is focused on the\nevaluation of the effect of sublethal doses of pesticides on solitary bees employing the OMICs\napproach. In the risk assessment of pesticides, the honey bee has long been preferred as a model\nspecies of pollinator, while solitary bees stay in the background. The methodology contains\ninnovative aspects for experiments, such as a modified isolator, preparation of plants for exposure,\nthe timing of the experiment, monitoring of active substance distribution or proteomic analysis of\nsolitary bees to identify hidden effects of the test substance or its formulation. In this methodology,\nthe model of the solitary bee, red mason bee (Osmia bicornis), is considered. However, the\nmethodology is also applicable to other, especially related species of solitary bees. The\nmethodology can be used in areas of state administration, private laboratories and research\nactivities in the assessment of environmental risks of pesticides on solitary bees. The\nmethodological procedure can confirm or even eliminate environmental risks when registering new\nproducts or reevaluating existing products. This methodology therefore has the potential for use in\ntesting new substances intended for plant protection before their registration. In an exemplary\nembodiment, the effect of acetamiprid in a Careo sticks formulation was tested. The distribution of\nacetamiprid in the soil substrate and the plant was evaluated. The hazardous metabolite\nacetamiprid IM-2-1 was identified in plant tissues. However, proteomic analysis indicated a very\nlow risk of acetamiprid for O. bicornis. This result is in agreement with the fact that acetamiprid\nhas been assessed by EFSA to be a low risk for bees and is registered in the EU until 28 February\n2033.
|
|
|
Current options for use of metabolomics in plant production
Maršík, Petr ; Vaněk, Tomáš
Metabolomics like other so-called "omics" approaches is based on the characterization and treatment as far as possible in the given dataset biological system. Meaning of each "omics" techniques lies mainly in their mutual integration which leads to the so-called. Systems biology linking output at the level of gene expression, protein translation and file metabolites with genomic data. These relationships are summarized in Fig. 1. The primary goal of systems biology is to understand the biological processes to such an extent that it is possible to predict the function of biological systems from the level of genes through metabolism, physiological processes to growth and development.
|
guest ::
